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BioChain是高質(zhì)量加工生物樣品產(chǎn)品和分析服務(wù)的先進者，用于推進轉(zhuǎn)化醫(yī)學(xué)，伴隨診斷和臨床產(chǎn)品研發(fā)。BioChain的產(chǎn)品可用于DNA和RNA測序，PCR和RT-PCR，基因表達分析，DNA和RNA純化，蛋白質(zhì)提取和純化以及蛋白質(zhì)表達分析。BioChain的下一代測序一站式服務(wù)，從臨床樣品采集，樣品制備，測序到生物信息學(xué)分析，將*改變行業(yè)。
除了我們*的生物標(biāo)本和樣品制備業(yè)務(wù)，BioChain正在進入臨床應(yīng)用領(lǐng)域，為癌癥和生殖健康提供臨床基因組學(xué)診斷產(chǎn)品和臨床試驗服務(wù)。BioChain致力于通過持續(xù)流程改進計劃以競爭力的價格實現(xiàn)高質(zhì)量的產(chǎn)品和服務(wù)。BioChain建立了全面的質(zhì)量保證計劃，以滿足客戶的滿意度，并為我們的內(nèi)部生產(chǎn)設(shè)施保持ISO 9001：2015和ISO 13485：2003認(rèn)證。
概觀
去除引物，酶，短DNA段，鹽和其他緩沖液成分可提高從PCR片段獲得的DNA序列數(shù)據(jù)的質(zhì)量。BioChain的SeqPure提供了一種，方便且經(jīng)濟的方法來清除長度超過100bp的DNA。
描述
SeqPure™PCR試劑是一種基于順磁珠技術(shù)的后PCR和下一代文庫制備清洗系統(tǒng)，旨在有效純化PCR擴增子。純化包括除去鹽，引物，引物二聚體，dNTP，因為DNA段選擇性地與磁珠顆粒結(jié)合; 用低鹽洗脫緩沖液或水洗脫高純度的DNA，可直接用于下游應(yīng)用。該協(xié)議可以使用您當(dāng)前的協(xié)議適應(yīng)您當(dāng)前的液體處理工作站（例如Beckman，Hamilton，Tecan，Caliper，Perkin Elmer，Agilent和Eppendorf），也可以手動執(zhí)行。
•PCR•突變檢測和基因分型•測序（Sanger和下一代）•微陣列•限制酶清除•克隆•片段分析
•與主要競爭對手的性能相似，價格低廉•在半小時內(nèi)凈化PCR產(chǎn)品•去除鹽，dNTP，引物和酶•無需離心或真空過濾•無花式緩沖液; 只需使用乙醇和Tris-HCI / TE•易于自動化•適用于96孔和384孔格式•可擴展：只使用盡可能多的珠子
用SeqPure珠和Ampure珠純化等量的含有鹽和蛋白質(zhì)的100bp梯子。使用不同的緩沖液TE和預(yù)熱的TE（用于增加產(chǎn)率）進行洗脫。加載洗脫的DNA（各8μl）并在1％瓊脂糖凝膠上電泳。凝膠顯示出3000-100bp的所有片段的優(yōu)異回收率。
SeqPure和AmPure在純化含有鹽和蛋白質(zhì)的100 bp DNA梯中的比較。通過分光光度法測量純度。260/280和260/230吸光度比均表明SeqPure可有效去除鹽和蛋白質(zhì)，與AmPure相當(dāng)。
對于許多分子生物學(xué)技術(shù)，從DNA中去除污染物對下游應(yīng)用至關(guān)重要。例如，為了確保成功的測序反應(yīng)，必須從模板中除去所有過量的污染物，例如引物，短缺失的PCR產(chǎn)物，未摻入的dNTP，鹽和殘留的蛋白質(zhì)。這些試劑可以干擾后續(xù)反應(yīng)并導(dǎo)致不可讀的序列。雖然去除多余的污染物是產(chǎn)生高質(zhì)量數(shù)據(jù)的關(guān)鍵，但這樣做可能非常耗時。PCR純化試劑盒可以方便地清除DNA，可以顯著提高實驗室的生產(chǎn)率?？梢允謩邮褂玫男颖咎准?，同時可以在各種平臺上輕松實現(xiàn)自動化，確保所有樣品通量都采用相同的程序 - 確保一致的性能。這樣的試劑盒也應(yīng)該是有效的，并且以小的損失純化DNA段。后，它應(yīng)該具有成本效益。 BioChain的SeqPure PCR純化試劑盒專為快速有效純化PCR產(chǎn)物而設(shè)計。該系統(tǒng)利用順磁珠技術(shù)選擇性地結(jié)合100bp和更大尺寸的DNA段。在洗脫純化的PCR產(chǎn)物之前，簡單的洗滌步驟將除去鹽和其他緩沖液成分，引物，引物二聚體，核苷酸和酶。SeqPure避免使用旋轉(zhuǎn)柱和其他純化膜，以便使試劑盒便于手動使用或自動化廣泛的實驗室自動化選擇（例如KingFisher，Beckman，Hamilton，Tecan，Caliper，Perkin Elmer，Agilent和Eppendorf）中。96-和384-井格式可以很容易地適應(yīng)這種技術(shù)。用順磁顆粒而不是旋轉(zhuǎn)柱進行純化可以減少水動力剪切 - 提高較大DNA段的完整性。順磁珠技術(shù)的可擴展性也使該套件更加靈活。如果您正在凈化少量DNA，您可以使用更少的珠子，并為每個純化試劑盒提取更多樣品。
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L5011001 | PureSil-Silica Magnetic Beads | 1 L | biochain |
L5011010 | PureSil-Silica Magnetic Beads | 10 mL | biochain |
Z1322006 | AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria | 1 kit (48 extractions) | biochain |
Z1322002 | AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml | 1 kit (48 extractions) | biochain |
Z1322001 | AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl | 1 kit (48 extractions) | biochain |
Z1322005 | AnaPrep Cultured Cell DNA Extraction Kit (48) For extracting genomic DNA from up to 5×105 cultured cells | 1 kit (48 extractions) | biochain |
Z1322009 | AnaPrep FFPE DNA Extraction Kit (48) For extracting genomic DNA from formalin-fixed paraffin-embedded tissue (FFPE) samples | 1 kit (48 extractions) | biochain |
Z1322010 | AnaPrep Forensic DNA Extraction Kit (48) For extracting genomic DNA from a wide range of forensic and human identity samples | 1 kit (48 extractions) | biochain |
Z1322007 | AnaPrep HPV DNA Extraction Kit for swab samples (48) For extracting HPV DNA from swab samples | 1 kit (48 extractions) | biochain |
Z1322008 | AnaPrep TB DNA Extraction Kit (48) For extracting Mycobacterium tuberculosis DNA from sputum | 1 kit (48 extractions) | biochain |
Z1322004 | AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains | 1 kit (48 extractions) | biochain |
Z1322015 | AnaPrep Total RNA Extraction Kit | 1 kit | biochain |
Z1322017 | AnaPrep Total RNA Extraction Kit with DNase-Treatment | 1 kit | biochain |
Z1322003 | AnaPrep Viral Nucleic Acid Extraction Kit (48) For extracting viral nucleic acids from plasma | 1 kit (48 extractions) | biochain |
Z1322011 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit A | 1 kit | biochain |
Z1322012 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit B | 1 kit | biochain |
T2235090-SB | CancerSeq™ Paraffin Tissue Tumor Block: Colon | 1 Block | biochain |
T2235152-SB | CancerSeq™ Paraffin Tissue Tumor Block: Lung | 1 Block | biochain |
T2235086-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Breast | 5 Slides | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235086-SB | CancerSeq™ Paraffin Tissue Tumor Block: Breast | 1 Block | biochain |
T2235218A-SB | CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma | 1 Block | biochain |
T2235090-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Colon | 5 Slides | biochain |
T2235152-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Lung | 5 Slides | biochain |
T2235218A-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Skin Melanoma | 5 Slides | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L1271010 | T4 RNA Ligase 1 | 1000 Units | biochain |
L1271050 | T4 RNA Ligase 1 | 5000 Units | biochain |
L1271500 | T4 RNA Ligase 1 | 50000 Units | biochain |
L1270010 | T4 RNA Ligase 2, truncated | 1000 Units | biochain |
L1270500 | T4 RNA Ligase 2, truncated | 50000 Units | biochain |
KS072012-I | Aligner 1-12 | 12 | biochain |
KS072012-II | Aligner 13-24 | 1 Kit | biochain |
KS072012-III | Aligner 25-36 | 1 Kit | biochain |
KS072012-IV | Aligner 37-48 | 1 Kit | biochain |
KS071012-12 | Gel Breaker Tubes | 12 | biochain |
KS071012-11 | Gel Cutter | 12 | biochain |
KS071012-13 | Gel Filter | 12 | biochain |
KS073012-I | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS073012-II | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS073012-III | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS073012-IV | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS073012 | RapidSeq High Yield Directional mRNA Sample Prep Kit – Without Aligner | 1 Kit | biochain |
KS073012-T | RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
KS074012-I | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS074012-II | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS074012-III | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS074012-IV | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS074012 | RapidSeq High Yield Small RNA Sample Prep Kit – Without Aligners | 1 Kit | biochain |
KS074012-T | RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
K5017100 | Blood and Serum DNA Isolation Kit | 1 kit | biochain |
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
K5019100 | FFPE Tissue DNA Extraction Kit | 1 kit | biochain |
K5011350 | FFPE Tissue DNA Extraction Kit – Column | 1 kit | biochain |
K5011450 | FFPE Tissue DNA Extraction Kit — Magnetic Beads | 1 kit | biochain |
K5016005 | Genomic DNA Extraction Kit | 1 kit | biochain |
K5011050 | Saliva DNA Isolation Kit | 1 kit | biochain |
K5018100 | Serum DNA Isolation Kit | 1 kit | biochain |
K5011250 | Stool DNA Isolation Kit | 1 kit | biochain |
K5011150 | Urine DNA Isolation Kit | 1 kit | biochain |
Z5060009 | xMag-Streptavidin Microparticles | 1 ml | biochain |
T6234701-1 | FDA Standard Frozen Tissue Array – Human Adult Normal | 2 slides | biochain |
T6234701-2 | FDA Standard Frozen Tissue Array – Human Adult Normal | 4 slides | biochain |
T6334701-1 | FDA Standard Frozen Tissue Array – Mouse Normal | 2 slides | biochain |
T6334701-2 | FDA Standard Frozen Tissue Array – Mouse Normal | 4 slides | biochain |
T6434701-1 | FDA Standard Frozen Tissue Array – Rat Normal | 2 slides | biochain |
T6434701-2 | FDA Standard Frozen Tissue Array – Rat Normal | 4 slides | biochain |
T6235201 | Frozen Tissue Array – 24 Different Prostate Tumors and 4 Corresponding Normal Controls | 1 slide | biochain |
T6235086-1 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235086-5 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235090-1 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235090-5 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235142-1 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235142-5 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235183-1 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235183-5 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235152-1 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 1 slide | biochain |
T6235152-5 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 5 slides | biochain |
T6734700-G02-1 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G02-5 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6734700-G01-1 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G01-5 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6234700-1 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6234700-5 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6235700-1 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6235700-5 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G02-1 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G02-5 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G01-1 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G01-5 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6134035-1 | Multiple Species Frozen Tissue Array – Brain | 1 slide | biochain |
T6134035-5 | Multiple Species Frozen Tissue Array – Brain | 5 slides | biochain |
T6134149-1 | Multiple Species Frozen Tissue Array – Liver | 1 slide | biochain |
T6134149-5 | Multiple Species Frozen Tissue Array – Liver | 5 slides | biochain |
上海起發(fā)實驗試劑有限公司是實驗試劑一站式采購服務(wù)商
1：強大的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。
2：產(chǎn)品種類齊全，經(jīng)營超過700多個品牌，基本涵蓋所有生物實驗試劑耗材。
3：提供加急服務(wù)，貨品一般1-2周到貨。
4：富有競爭力的價格優(yōu)勢，絕大部分價格有優(yōu)勢。
5：多年積累良好的信譽，大部分客戶提供貨到付款服務(wù)。客戶包括清華、北大、交大、復(fù)旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批發(fā)，歡迎合作。
地址：上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
郵箱：xs1@78bio.com
傳真：021-50724961
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